NEB offers a variety of interactive tools to help you accurately design primers for your specific needs.

Optimal primer sequences and appropriate primer concentrations are essential for maximal specificity and efficiency in PCR. The table, Primer design and usage guidelines, provides an …

Design PCR primers online with Primer3. Specify target regions, melting temperatures, GC content, and product sizes. Get optimal primer pairs for your DNA sequences.

Short primers are mainly used for amplifying a small, simple fragment of DNA. On the other hand, a long primer is used to amplify a eukaryotic genomic DNA sample.

Enhancements and modifications of primer design program Primer3. Bioinformatics 23 (10):1289-91.

Streamline your workflow with our online Primer Designer Tool to search for the right PCR and Sanger sequencing primer pair from a database of ~650,000 predesigned primers.

Primerize is a Web Server for primer designs of DNA sequence PCR assembly. Primerize is optimized to reduce primer boundaries mispriming, is designed for fixed sequences of RNA …

Whether you're conducting gene expression studies, diagnostic testing, or molecular cloning, our tool helps you design primers that meet your specific requirements.

Click on the "Design Primers" button to get a list of appropriate PCR primer pairs. The output includes a proposed annealing temperature for each listed primer pair.

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts.